Abstract: For the problem of divorced from industry, divorced from profession education and lack of cross-specialty cooperation training in the application-oriented university innovative and entrepreneurial talent training at present, the new training mode of “Internet plus” innovative and entrepreneurial talent of cross-specialty integration, industry-university-research-innovation integration is proposed. The innovation of the mode is carrying out reform and innovation in the curriculum system, practical teaching system, teaching way and quality evaluation system, to push forward the industry and education integration, the specialty and innovation integration, the cross-specialty integration, to solve the above problems and improve the quality of innovative and entrepreneurial talent training with the production chain of “Internet plus” enterprises(Product Prototype Design → Programming Development → Marketing/Operations) as the main line.