Abstract: As a long-term systematic project, the cultivation of outstanding innovative talents in biotechnology needs to follow the laws of talent growth and the sustainable development of the times. The teaching team of the biotechnology core curriculum group is based on the national“101 Plan”strategic orientation, relying on knowledge graph technology to reconstruct the teaching system of the core curriculum group. Through multi-dimensional teaching mode innovation, it breaks down the cognitive barriers of the curriculum, constructs a vertically connected and horizontally related knowledge network, avoids knowledge fragmentation, establishes a“knowledge ability literacy”transformation mechanism, strengthens the ability to solve complex problems, meets the requirements of the new engineering construction, and constructs a composite ability matrix of outstanding talents in biotechnology.

Key words: “101 Plan”, Knowledge graph, Core course group, Biotechnology, “Chief engineer type”talent, Teaching reform