Abstract: Biological separation technology is an essential downstream key technology in biotechnology that separates target products from plant cells, fermentation broth, and enzyme reaction broth. It is widely used in fields such as food and medicine. Biological separation technology is a core professional course for master's students majoring in biology and medicine, which plays a crucial role in talent cultivation in the pharmaceutical industry. Given the problems of emphasizing theory over practice, single teaching methods, and poor teaching effectiveness in traditional teaching, this article conducts systematic reform research from multiple dimensions, including optimizing course teaching objectives, reconstructing teaching content, innovating teaching methods, and optimizing evaluation mechanisms. The focus is on cultivating composite professional talents who possess both practical skills and theoretical literacy, as well as the ability to solve complex technical problems.

Key words: Biological separation technology, Biology and medicine, Reform in teaching, Graduate student, Teaching objectives, Content of courses