Abstract: This study explores integrated laboratory construction for new business and AI education, addressing research gaps with an ecosystem theory-based framework and implementation path. It designs functional modules, technical solutions, and platform architecture, validated at a university. Results show improved teaching, research, multidisciplinary collaboration, and talent cultivation. It supports educational transformation theoretically and guides laboratory development and industry-academia-research integration practically.

Key words: New business education, Artificial intelligence education, Integrated construction, Laboratory ecosystem, Versatile talent cultivation, Industry-academia-research integration