真菌基因组组装技术在《农业微生物学》开放实验中的设计与实践

创新创业理论研究与实践 ›› 2022, Vol. 5 ›› Issue (15): 182-184.

真菌基因组组装技术在《农业微生物学》开放实验中的设计与实践

李诚, 杨再福, 王勇

贵州大学农学院植物病理学教研室,贵州贵阳 550025

出版日期:2022-08-10 发布日期:2022-10-29
通讯作者: 王勇(1980-),男,山东泰安人,博士,教授,研究方向：植物病理学教学科研工作,通讯邮箱：457329415@qq.com。
作者简介:李诚(1988-),男,彝族,云南凤庆人,博士,副教授,研究方向：植物病理学教学科研工作。
基金资助:
贵州省科技计划基础研究项目（黔科合基础-ZK〔2021〕一般142）; 贵州大学引进人才科研项目（贵大人基合字〔2020〕 35号）; 贵州大学培育项目（贵大培育〔2020〕24号和贵大培育〔2019〕13号）

Design and Practice of Fungal Genome Assembly Technology in an Open-Ended Experiment of Agricultural Microbiology

LI Cheng, YANG Zaifu, WANG Yong

Department of Plant Pathology, College of Agriculture, Guizhou University, Guiyang Guizhou, 550025, China

Online:2022-08-10 Published:2022-10-29

摘要/Abstract

摘要： 通过基因组组装技术获得高质量的基因组信息是开展真菌基因组学研究的前提。近年来,该技术在微生物研究领域应用广泛。为在本科教学中推广该技术,贵州大学农学院在院级平台课《农业微生物学》中设计开放实验,让植物保护本科专业的学生分组实践。具体实验内容为以HGAP4、Canu和MECAT对天麻褐腐病菌Fusarium oxysporum测序数据的基因组进行组装,并通过QuickMerge软件整合后得到最终的基因组序列,通过实践提高学生对微生物基因组研究的兴趣及动手能力,进而丰富教学内容,提高教学质量。

关键词: 基因组组装, 《农业微生物学》, 开放实验, 组装软件, 实验分析, 实践

Abstract: Obtaining high-quality genomic information through genome assembly technology is the premise of fungal genomic research. In recent years, this technology has been widely used in the genomic research of microorganisms. In order to popularize this technology in undergraduate teaching, the College of Agriculture of Guizhou University used the college-level course Agricultural Microbiology to devise an open-ended experiment for students of undergraduate majors of plant protection. The experiment included the use of HGAP4, Canu, and MECAT to assemble the genome of Fusarium oxysporum. Data sequencing was carried out followed by integration with QuickMerge to obtain the final genome sequence. The open-ended experiment module described herein may greatly improve students' interest in microbial genome research, and their skills and knowledge related to the same, by enriching the teaching content and improving the quality of teaching.

Key words: Genome assembly, Agricultural Microbiology, Open experiments, Assembly software, Experimental analysis, Practice

中图分类号:

G642

李诚, 杨再福, 王勇. 真菌基因组组装技术在《农业微生物学》开放实验中的设计与实践[J]. 创新创业理论研究与实践, 2022, 5(15): 182-184.

LI Cheng, YANG Zaifu, WANG Yong. Design and Practice of Fungal Genome Assembly Technology in an Open-Ended Experiment of Agricultural Microbiology[J]. The Theory and Practice of Innovation and Enntrepreneurship, 2022, 5(15): 182-184.

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